About the project
The project started in 2007, when local varieties of soybean (Glycine max [L.] Merr.; variety Soniachna) and flax (Linum ussitatissimum, L.; variety Kyivskyi) were sown to non-radioactive and radioactive experimental fields established in the Chernobyl area. Developing and mature seeds are harvested on yearly basis, analyzed and remaining mature seeds are sown in order to obtain next generation seeds. The method used for the analysis is proteomics based on protein two-dimensional gel electrophoresis (2-DE) and tandem mass spectrometry (MS/MS). Firstly, proteins are isolated from harvested seeds and separated using 2-DE. This method separate proteins in first dimension based on isoelectric point (pI) and in second dimension based on molecular weight (MW).
Schematic view of 2-DE. The picture also show instrumentations used in this project.
Then protein spots presented on 2-DE gels are analyzed by computer software and protein abundances are determined. Then, spots are excised from 2-DE gels and analyzed by tandem mass spectrometry (MS/MS) for protein identification.
Mass spectrometer used in this project.
As the output of this analysis, the abundances for hundreds of proteins presented in developing and mature seeds are obtained. These data are used to characterize alterations in protein abundances that are presented during seed development in radioactive Chernobyl area.