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Flax - second generation | Seeds in Chernobyl

Flax - second generation

Interactive 2-DE gel of proteins in mature flax seeds harvested from RA and NRA Chernobyl fields

The aim of this experiment was to identify differentially abundant proteins during flax seed development in Chernobyl radioactive (RA) and non-radioactive (NRA) experimental fields. Flax seeds were harvested at 2, 4, and 6 weeks after flowering (WAF) and in mature seeds (M) from both fields. Protein 2-DE gels from NRA and RA Chernobyl fields were analyzed separately using ImageMaster software 4.9. Gels of each developmental stage were matched individually to the reference gel in biological triplicate. A reference gel was obtained by analysis of pooled sample from all investigated stages of seed development. All matched spots were grouped into subclasses. Developmental profiles for a particular 2-DE spot were formed from spot subclasses across all developmental stages.

To ensure high quality of the datasets for comparative analysis, only 2-DE spot that satisfied the following criteria were included: 1) a 2-DE spot must be present in both datasets (i.e. from radioactive and non-radioactive field); 2) in each dataset, a 2-DE spot must be present in at least in two biological replicates and in at least three developmental stages. A total 199 2-DE spots satisfied these thresholds are circled on interactive 2-DE gel. The volumes of all spots that satisfied these criteria were normalized as relative volumes (%V) to compensate for small differences in sample loading or gels staining. Proteins in 79 2-DE spots (out of 199) were identified using tandem mass spectrometry.

Cite these data:

Klubicová K, Danchenko M, Skultety L,  Berezhna VV,  Rashydov NM, Hajduch M. 2013. Radioactive Chernobyl Environment Has Produced High-Oil Flax Seeds That Show Proteome Alterations Related to Carbon Metabolism during Seed Development. J Proteome Res. 12(11):4799-4806