Flax - second generation
The aim of this experiment was to identify differentially abundant proteins during flax seed development in Chernobyl radioactive (RA) and non-radioactive (NRA) experimental fields. Flax seeds were harvested at 2, 4, and 6 weeks after flowering (WAF) and in mature seeds (M) from both fields. Protein 2-DE gels from NRA and RA Chernobyl fields were analyzed separately using ImageMaster software 4.9. Gels of each developmental stage were matched individually to the reference gel in biological triplicate. A reference gel was obtained by analysis of pooled sample from all investigated stages of seed development. All matched spots were grouped into subclasses. Developmental profiles for a particular 2-DE spot were formed from spot subclasses across all developmental stages.
To ensure high quality of the datasets for comparative analysis, only 2-DE spot that satisfied the following criteria were included: 1) a 2-DE spot must be present in both datasets (i.e. from radioactive and non-radioactive field); 2) in each dataset, a 2-DE spot must be present in at least in two biological replicates and in at least three developmental stages. A total 199 2-DE spots satisfied these thresholds are circled on interactive 2-DE gel. The volumes of all spots that satisfied these criteria were normalized as relative volumes (%V) to compensate for small differences in sample loading or gels staining. Proteins in 79 2-DE spots (out of 199) were identified using tandem mass spectrometry.
Cite these data:
Klubicová K, Danchenko M, Skultety L, Berezhna VV, Rashydov NM, Hajduch M. 2013. Radioactive Chernobyl Environment Has Produced High-Oil Flax Seeds That Show Proteome Alterations Related to Carbon Metabolism during Seed Development. J Proteome Res. 12(11):4799-4806